Two-Dimensional Gel Electrophoresis Analysis of the Response of Pseudomonas putida KT2442 to 2-Chlorophenol

Two-Dimensional Gel Electrophoresis Analysis of the Response of Pseudomonas putida KT2442 to 2-Chlorophenol.

The effects of exposure of Pseudomonas putida KT2442 to 2-chlorophenol as a model for the chemical stress response were examined by two-dimensional polyacrylamide gel electrophoresis. Individual protein concentrations were determined at 45, 65, and 95 min following the addition of 2-chlorophenol at a concentration of 1.63 mM to exponentially growing cultures of P. putida KT2442 by silver staini...

Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins.

The responses of Pseudomonas putida KT2442 to various forms of nutrient starvation and stress conditions were examined by two-dimensional polyacrylamide electrophoresis. Carbon deprivation resulted in a temporal expression of two classes of starvation-induced proteins: one class was transiently expressed during the initial phase of starvation, and the second class was expressed throughout the e...

Salmonella typhimurium LT-2, Pseudomonas putida KT2442 and Rhizobium meliloti

mhpT Encodes an Active Transporter Involved in 1 3-(3-Hydroxyphenyl)propionate Catabolism by Escherichia coli K-12 2 3 Ying Xu, Bing Chen, Hongjun Chao and Ning-Yi Zhou 4 5 Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of 6 Virology, Chinese Academy of Sciences, Wuhan 430071, China 7 8 *Corresponding author: Ning-Yi Zhou. 9 Email: [email protected]. 10 ...

Two-Dimensional Gel Electrophoresis (2-DE)

Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and Sodium Dodecyl Sulphate PolyAcrylamide-Gel Electrophoresis (SDS-PAGE) in second dimension. This particular configuration is called classical 2-DE: IEF separates proteins in function of their isoelectric point (pI) and SDS-...

Two-dimensional Gel Electrophoresis